A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species
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منابع مشابه
A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species.
The aim of the present study was to establish a protocol of sperm cryopreservation in Misgurnus anguillicaudatus and verify the applicability of the obtained protocol in other loach species. We evaluated the following parameters: inseminating dose, thawing temperatures (20, 25 and 30 degrees C for 10s), extenders (loach or cyprinid extenders), internal cryoprotectants (dimethyl sulfoxide (DMSO)...
متن کاملMorphometric and Histological Changes in Cyprinid Loach, Misgurnus anguillicaudatus, in the Early Growth Period
In this study, we measured the morphometric and histological changes in the cyprinid loach, Misgurnus anguillicaudatus, during the early period of growth. Eyes, yolk length, yolk height, and yolk volume of the larva decreased for 16 days post hatching (DPH) (P<0.05). During 60 DPH (P>0.05), the most anterior extension of the head × the posterior end of the supraoccipital, the most anterior exte...
متن کاملAnalysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus anguillicaudatus
In this study, we selected natural polyploidy loach (diploid, triploid and tetraploid) and hybrid F₁ generation obverse cross (4 × 2) and inverse cross (2 × 4) by diploids and tetraploids as the research model. The MSAP (methylation-sensitive amplified polymorphism) reaction system was established by our laboratory to explore methylation levels and pattern diversification features at the whole ...
متن کاملComparative Analysis of Mitochondrial Genomes in Distinct Nuclear Ploidy Loach Misgurnus anguillicaudatus and Its Implications for Polyploidy Evolution
Misgurnus anguillicaudatus has several natural ploidy types. To investigate whether nuclear polyploidy have an impact on mitochondrial DNA (mtDNA), the complete mitochondrial genomes (mitogenomes) of five distinct ploidy M. anguillicaudatus (natural diploid, triploid, tetraploid, pentaploid and hexaploid), which were collected in central China, were sequenced and analyzed. The five mitogenomes ...
متن کاملFirst record of the natural occurrence of hexaploid loach Misgurnus anguillicaudatus in Hubei Province, China.
Natural occurrence of hexaploid loach Misgurnus anguillicaudatus detected in central China is reported here for the first time. The evidences from karyotyping, DNA content analysis and nuclear volume measurements were described to confirm the hexaploid nature of the identified individual.
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ژورنال
عنوان ژورنال: Animal Reproduction Science
سال: 2009
ISSN: 0378-4320
DOI: 10.1016/j.anireprosci.2009.02.021